Invention Grant
- Patent Title: Delivery, engineering and optimization of tandem guide systems, methods and compositions for sequence manipulation
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Application No.: US14972927Application Date: 2015-12-17
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Publication No.: US11008588B2Publication Date: 2021-05-18
- Inventor: Feng Zhang , Chie-yu Lin , Fei Ran
- Applicant: The Broad Institute, Inc. , Massachusetts Institute of Technology , President and Fellows of Harvard College
- Applicant Address: US MA Cambridge; US MA Cambridge; US MA Cambridge
- Assignee: The Broad Institute, Inc.,Massachusetts Institute of Technology,President and Fellows of Harvard College
- Current Assignee: The Broad Institute, Inc.,Massachusetts Institute of Technology,President and Fellows of Harvard College
- Current Assignee Address: US MA Cambridge; US MA Cambridge; US MA Cambridge
- Agency: Foley & Lardner LLP
- Main IPC: C12N15/90
- IPC: C12N15/90 ; C12N15/10 ; C12N9/22 ; C12N15/63
Abstract:
The invention provides for delivery, engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in prokaryotic and eukaryotic cells to ensure enhanced specificity for target recognition and avoidance of toxicity.
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