Method for high-throughput AFLP-based polymorphism detection
Abstract:
The invention relates to a kit for use in a method for detecting genetic variation in one or more members of a population, comprising (a) an adaptor adapted for ligation to a plurality of nucleic acid fragments and (b) a set of primers for PCR amplification having a 5′-end and a 3′-end, wherein at least one of the primers comprises one or more selective nucleotides at the 3′ end, and wherein the adaptor and/or at least one of the primers comprises a sample-specific identifier sequence capable of indicating sample origin of an amplification product.
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