The present disclosure relates to a display panel and a manufacturing method thereof, a display device. The display panel includes an array substrate and a color film substrate set oppositely, the array substrate includes multiple subpixel units arranged in an array and an orientation layer covering multiple subpixel units, each subpixel unit includes an opening area; the color film substrate is provided with support columns, orthographic projections of the support columns on the subpixel units are outside the opening areas, the orientation layer is formed with support protrusions which protrude from a surface of the orientation layer, orthographic projections of the support protrusions on the subpixel units are outside the opening areas, the support columns contact top ends of the support protrusions to prevent the support columns from contacting the surface of the orientation layer.