The disclosure discloses a method of calibrating a moving region of a guide robot, including: detecting and receiving a movement instruction, and sending the received movement instruction to a controller; determining region calibration parameters corresponding to the received movement instruction according to a predetermined mapping relation between the movement instruction and the region calibration parameters, wherein the region calibration parameters include a pending moving region of the guide robot, a light display parameter in the pending moving region, and a driving parameter corresponding to the pending moving region and the light display parameter; driving the light emitting module to carry out light display calibration in the pending moving region according to the determined region calibration parameters. The disclosure further provides the guide robot and a computer storage medium. The technical solution of the disclosure enables the guide robot to display a marked region to be passed.