Invention Grant
- Patent Title: Linked-fragment sequencing
-
Application No.: US16088717Application Date: 2017-03-28
-
Publication No.: US11021742B2Publication Date: 2021-06-01
- Inventor: Andrea Marziali , Joel Pel
- Applicant: Boreal Genomics, Inc.
- Applicant Address: CA Vancouver
- Assignee: Boreal Genomics, Inc.
- Current Assignee: Boreal Genomics, Inc.
- Current Assignee Address: CA Vancouver
- Agency: Brown Rudnick LLP
- International Application: PCT/IB2017/051778 WO 20170328
- International Announcement: WO2017/168331 WO 20171005
- Main IPC: C12Q1/6855
- IPC: C12Q1/6855 ; C12Q1/686 ; C12Q1/6806 ; C12Q1/6874 ; C40B20/04 ; C12Q1/6869 ; C12Q1/6853 ; C40B40/08 ; C12Q1/6876 ; C12Q1/6886

Abstract:
The invention generally relates to sequencing library preparation methods. In certain embodiments, two or more template nucleic acids are joined together by a linking molecule, such as a PEG derivative. Identical copies of a nucleic acid fragment or both strands of a duplex fragment may be linked together. The linked nucleic acids are amplified, creating linked amplicons. Emulsion PCR with linked primers creates linked template nucleic acids for seeding sequencing clusters and errors can be readily identified by their presence on only one of the linked fragments.
Public/Granted literature
- US20190127788A1 LINKED-FRAGMENT SEQUENCING Public/Granted day:2019-05-02
Information query
IPC分类: