The present disclosure is directed to a method of assembly of a rotor blade for a wind turbine. The method includes placing a first rotor blade section onto a first set location of an assembly fixture, wherein the first rotor blade includes a first locating datum such that the assembly fixture at the first set location constrains movement of the first rotor blade section at the first locating datum along a first direction; placing the first rotor blade section onto a second set location of the assembly fixture, wherein the first rotor blade includes a second locating datum such that the assembly fixture at the second set location constrains movement of the first rotor blade section at the second locating datum along a second direction; and positioning a second rotor blade section onto the first rotor blade section within the assembly fixture.