Versatile, concise and convenient process for the commercial scale preparation of highest pure 3,3′5,5′-tetramethylbenzidine (TMB) and its salts, a chromogenic substrate used in staining procedures in immunohistochemistry and visualizing reagent in enzyme-linked immunosorbent assays
Abstract:
The present invention relates to a process for the preparation of 3,3′,5,5′-Tetramethylbenzidine represented by formula I, and processes for the preparation of intermediates used in the preparation of 3,3′,5,5′-Tetramethylbenzidine.
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