A bathroom cleaning assembly for cleaning a bathroom includes a housing. A handle is slidably coupled to the housing for gripping. A pair of wheels is each rotatably coupled to the housing for rolling along a support surface. A plurality of dispensing units is provided and each of the dispensing units is positioned in the housing. Moreover, each of the dispensing units contains a fluid. A plurality of brushes is provided and a selected one of the brushes is selectively and fluidly coupled to a selected one of the dispensing units. The selected brush dispenses the fluid from the selected dispensing unit for cleaning the bathroom. A vacuum unit is positioned in the housing for vacuuming debris from the bathroom.