A needle guide holder, which is used for a fixing medical puncture needle and a ultrasonic probe, comprises a holder body (1a, 1b) and a fixing device (2a, 2b) used in cooperation with the holder body (1a, 1b). The fixing device (2a, 2b) is mounted onto the holder body (1a, 1b) and provided with a weakening portion (23a, 23b). The needle guide holder is designed to be disposable, thereby avoiding the possibility of cross-infection caused by using the same needle guide holder on different patients. The needle guide holder can be used on one and same patient to perform a number of treatments just through the replacement of a sliding frame and without replacing the fixing device and a fixing frame. In a treatment process, the puncture needle is left in the body for a subsequent treatment, while the needle guide holder and the ultrasonic probe can be removed from the human body.