The invention relates to an infant feeding assembly comprising a feeding bottle and a sealed cup holding a single serve of infant formula to be mixed with water. The bottle comprises a cup holder, a teat, a mounting ring, a water fill opening and a closure device. The cup holder is adapted to hold the cup, in an opened condition. The teat has a dome shaped base and a spout. The mounting ring releasably mounts the teat onto the cup holder. According to the invention, the water fill opening, through which a mixing cavity of the feeding bottle can be filled with water while the opened cup is held by the cup holder and the teat is mounted on the cup holder, is provided in the cup holder and/or in the mounting ring and/or in the teat. The cup and the teat at least partially define the mixing cavity.