A service provider provides virtual computing services using a fleet of one or more host computer systems. Each of the host computer systems may be equipped with a trusted platform module (“TPM”). The service provider, the host computer systems, and the virtual computing environments generate attestations that prove the integrity of the system. The attestations are signed with a one-time-use cryptographic key that is verifiable against the public keys of the service provider, a host computer system, and a virtual computing environment. The public key of the host computer system is integrated into a hash tree that links the public key of the host computer system to the public key of the service provider. The public key of the virtual computing environment is signed using a one-time-use graphic key issued to the host computer system that hosts the virtual computing environment.