Invention Grant
- Patent Title: CRISPR fluorescent guide RNA (fgRNA) to understanding gRNAs expressed from pol II promotors
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Application No.: US16632792Application Date: 2017-09-29
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Publication No.: US11041154B2Publication Date: 2021-06-22
- Inventor: Samira Kiani , Xiao Wang , David Menn , Mo Reza Ebrahimkhani
- Applicant: Arizona Board of Regents on behalf of Arizona State University
- Applicant Address: US AZ Scottsdale
- Assignee: Arizona Board of Regents on behalf of Arizona State University
- Current Assignee: Arizona Board of Regents on behalf of Arizona State University
- Current Assignee Address: US AZ Scottsdale
- Agency: Quarles & Brady LLP
- International Application: PCT/US2017/054271 WO 20170929
- International Announcement: WO2019/017988 WO 20190124
- Main IPC: C12N15/113
- IPC: C12N15/113 ; C12N9/22 ; C12N15/63
Abstract:
Provided herein are tools for understanding and engineering dynamics of synthetic genetic circuits which utilize CRISPR components. More particularly, methods, systems, and compositions for directing Cas9 activity using a fluorescent guide RNA (fgR-NA) which fluoresces in the presence of small molecules (e.g., DFHBI-IT) are described and illustrated in the present provisional application.
Public/Granted literature
- US20200377884A1 CRISPR Fluorescent Guide RNA (fgRNA) to Understanding gRNAs Expressed from Pol II Promotors Public/Granted day:2020-12-03
Information query
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