Invention Grant
- Patent Title: Optimization methods for making a synthetic gene
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Application No.: US15535760Application Date: 2015-12-21
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Publication No.: US11041158B2Publication Date: 2021-06-22
- Inventor: James R. Henriksen
- Applicant: AgBiome, inc.
- Applicant Address: US NC Research Triangle Park
- Assignee: AgBiome, inc.
- Current Assignee: AgBiome, inc.
- Current Assignee Address: US NC Research Triangle Park
- Agency: Womble Bond Dickinson (US) LLP
- International Application: PCT/US2015/067024 WO 20151221
- International Announcement: WO2016/106184 WO 20160630
- Main IPC: C12N15/67
- IPC: C12N15/67 ; G16B25/00 ; G16B30/00 ; C12N15/82 ; C07K14/325

Abstract:
Methods for making a synthetic gene are provided. The methods find use in optimizing a candidate gene nucleic acid sequence for expression in a selected target expression system. The method identifies stable or retained sequences in the candidate gene nucleic acid sequence, identifies disallowed sequences, develops a statistical model based on a whole genome, a partial genome, or transcriptome sequences of the target expression system, generates an optimized candidate gene nucleic acid sequence for use in the target expression system, and makes a synthetic gene comprising the optimized candidate gene nucleic acid sequence. The method allows for optimization of the candidate gene nucleic acid sequence without removing certain stable or retained sequences. Rather, the activity of these sites is positionally modulated or inactivated through upstream and downstream modifications of codons and/or sequence patterns.
Public/Granted literature
- US20170369890A1 METHODS FOR MAKING A SYNTHETIC GENE Public/Granted day:2017-12-28
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