Disclosed in the present application is a method and a device of estimating a timing location. The method includes: for each preamble subsequence, acquiring a detection window in which a peak value of a correlation value power of each preamble subsequence is located; accumulating the correlation value powers of all corresponding locations in the acquired detection window of each preamble subsequence, to obtain a cumulative sum of the correlation value powers corresponding to each location, wherein, in the accumulation, the correlation value powers of the preamble subsequence are within detection window in which peak values of respective correlation value powers are located; determining, according to the cumulative sum of the correlation value powers, peak power and noise power; and estimating, according to the peak power and the noise power, a timing location. The present application can solver the problem of a current M-stage preamble sequence solution in which a larger large frequency offset greatly impacts timing location estimation precision.