An eyeglasses assembly includes two lenses, a frame, and two positioning blocks. The frame includes two frame portions. Each frame portion includes upper and lower portions spaced from each other by a notch. The upper portion includes an insertion hole. The lower portion includes an engaging groove aligned with the insertion hole. At least one engaging hole extends from an outer surface of the lower portion to the engaging groove in a thickness direction. Each positioning block is mounted in an associated insertion hole and an associated engaging groove. Each positioning block includes at least one assembling hole to be aligned with the at least one engaging hole. Each of a plurality of fasteners extends through the at least one assembling hole of an associated one of the two positioning blocks and the at least one engaging hole of the associated lower portion.