A device substrate includes a first substrate, an active device array, a data line pad, a gate drive circuit test line, and a first barrier structure. The active device array is located on the first substrate. The data line pad is electrically connected to the active device array. The gate drive circuit test line is located on the first substrate. The first barrier structure is located on the first substrate. The first barrier structure is located between the gate drive circuit test line and the data line pad. The barrier structure includes a first blocking wall.