- Patent Title: Modified nucleotides for synthesis of nucleic acids, a kit containing such nucleotides and their use for the production of synthetic nucleic acid sequences or genes
-
Application No.: US15507853Application Date: 2015-09-01
-
Publication No.: US11059849B2Publication Date: 2021-07-13
- Inventor: Thomas Ybert , Sylvain Gariel
- Applicant: DNA Script
- Applicant Address: FR Le Kremlin-Bicêtr
- Assignee: DNA Script
- Current Assignee: DNA Script
- Current Assignee Address: FR Le Kremlin-Bicêtr
- Agency: Bozicevic, Field & Francis LLP
- Agent Edward J. Baba; Kathleen Y. Rao
- Priority: FR1458194 20140902
- International Application: PCT/FR2015/052310 WO 20150901
- International Announcement: WO2016/034807 WO 20160310
- Main IPC: C07H21/04
- IPC: C07H21/04 ; C07H19/10 ; C07H19/20 ; C12P19/34
Abstract:
A modified nucleotide, intended for the synthesis of long chain nucleic acids by enzymatic processes, comprising a “natural” nitrogenous base or a natural nitrogenous base analogue, a ribose or deoxyribose carbohydrate, and at least one phosphate group, characterized in that said nucleotide comprises at least one R group, termed the modifier group, carried by said nitrogenous base or analogue and/or by the oxygen in position 3′ of the ribose or deoxyribose molecule, making it possible to block the polymerization of said nucleotide and/or to allow the interaction of said nucleotide with another molecule, such as a protein, during the nucleic acid synthesis, R comprising at least one functional terminal group.
Public/Granted literature
Information query
