A barcode reader is provided. The barcode reader includes a first image acquisition assembly having a first imager assembly configured to capture infrared (IR) light and an IR illumination assembly configured to emit IR light over at least a portion of a first field of view (FOV) of the first imager assembly so as to illuminate targets within the first FOV. The barcode reader further includes a second image acquisition assembly having a second imager assembly configured to capture visible light and a visible-light illumination assembly configured to emit visible light over at least a portion of a second FOV of the second imager assembly so as to illuminate targets within the second FOV.