A chip-on-film package including a flexible substrate, first test pads, second test pads, first connecting wires, second connecting wires and a chip is provided. The flexible substrate includes at least one segment. Each segment has a central portion and a first side portion and a second side portion located at two opposite sides of the central portion. The chip disposed on the central portion includes first connecting pads and second connecting pads. The first test pads and the second test pads are disposed on the first side portion. Two ends of each of the first connecting wires are connected to the corresponding first connecting pad and the corresponding first test pad. Two ends of each of the second connecting wires are connected to the corresponding second connecting pad and the corresponding second test pad. Each of the second connecting wires includes a first section located at the second side portion.