Invention Grant
- Patent Title: High titer recombinant AAV vector production in adherent and suspension cells
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Application No.: US14915630Application Date: 2014-08-28
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Publication No.: US11078464B2Publication Date: 2021-08-03
- Inventor: Huiren Zhao , Grace Ki Jeong Lee , Thomas Wolfe , Cherylene Plewa , Jackie Z. Sheng
- Applicant: AMGEN INC.
- Applicant Address: US CA Thousand Oaks
- Assignee: AMGEN INC.
- Current Assignee: AMGEN INC.
- Current Assignee Address: US CA Thousand Oaks
- Agent Jonathan M. Dermott
- International Application: PCT/US2014/053281 WO 20140828
- International Announcement: WO2015/031686 WO 20150305
- Main IPC: C12N7/00
- IPC: C12N7/00 ; C12N15/86
Abstract:
The present invention is directed to an in vitro method of producing a recombinant AAV virion in a mammalian host cell that comprises a functional adenoviral E1A gene and rAAV virions made by the method. Host cells are transfected with varying ratios of plasmids comprising the E1A gene and virions.
Public/Granted literature
- US20160222356A1 High Titer Recombinant AAV Vector Production in Adherent and Suspension Cells Public/Granted day:2016-08-04
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