A filter set for simultaneously observing fluorescent and non-fluorescent object regions, and an observation system and a method in which the filter set is used are disclosed. The illumination and observation light filters of the filter set each have two separate passbands with high transmission, which are spectrally defined such that illumination light outside the emission spectrum of fluorescence can pass through the illumination and observation light filters and fluorescent light is not swamped by the illumination light. The transmission of the passbands is defined such that non-fluorescent object regions can be observed substantially with color fidelity.