The present invention provides a substrate and a manufacturing method of the substrate. The substrate includes: a glass base plate; a first base layer on the glass base plate; a light shielding layer on the first base layer; multiple pixel units placed on the first base layer, the pixel units including at least one light transmissive region; and a photomask for exposing the light shielding layer. The photomask is used to expose, develop, and etch away a portion of the light shielding layer on the pixel units which need to communicate with each other, so that multiple grooves are formed in the light shielding layer to make the pixel units communicate with each other.