The present disclosure provides a signal calibration method, apparatus and device generated based on an imbalance of I path and Q path. The method includes sending a cosine signal and a sine signal through a signal generator, transmitting the cosine signal and the sine signal in the I path and the Q path respectively, the cosine signal and the sine signal being configured to loop back to a signal receiving direction after passing through a transmitting amplifier; processing a signal obtained by a down converter in the signal receiving direction; performing a phase adjustment and an amplitude adjustment by adjusting the signal generator, gain amplifiers of I path and Q path analog domains, and a corresponding digital domain, so as to determine an appropriate phase cancellation value and an appropriate amplitude cancellation value for an image signal; and calibrating the image signal corresponding to the signal to be calibrated.