Invention Grant
- Patent Title: Bacterial strain producing 2,3-butanediol and other metabolites
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Application No.: US16316961Application Date: 2017-07-18
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Publication No.: US11111474B2Publication Date: 2021-09-07
- Inventor: Tomás Roncal Martínez , Susana Caballero Román , María Del Mar Díaz De Guereñu Zabarte , Inés Rincón Arroyo , Soraya Prieto Fernández , José Ramón Ochoa Gómez
- Applicant: FUNDACION TECNALIA RESEARCH & INNOVATION
- Applicant Address: ES Donostia-San Sebastián
- Assignee: FUNDACION TECNALIA RESEARCH & INNOVATION
- Current Assignee: FUNDACION TECNALIA RESEARCH & INNOVATION
- Current Assignee Address: ES Donostia-San Sebastián
- Agency: Squire Patton Boggs (US) LLP
- Priority: EP16382347 20160719
- International Application: PCT/EP2017/068117 WO 20170718
- International Announcement: WO2018/019656 WO 20180201
- Main IPC: C12N1/20
- IPC: C12N1/20 ; C12P7/26 ; C12P7/56 ; C12N15/03 ; C12P7/16 ; C12P7/18 ; C12N15/01 ; C12R1/46

Abstract:
The present invention provides a modified strain of the bacterium Lactococcus lactis obtainable by a method that comprises a step of fusion of two protoplasts from two Lactococcus lactis parental strains which, compared to the wild type strain of Lactococcus lactis from which they derive, show: (a) an increased ability to produce acetoin and/or 2,3-butanediol (2,3-BDO), and (b) a decreased ability to produce lactic acid, when cultured under aerobic conditions, and wherein the modified strain of Lactococcus lactis has an increased ability to produce 2,3-BDO of at least 20 times the amount produced by the wild type strain, an increased ability to produce acetoin of at least 20 times the amount produced by the wild type strain and a decreased ability to produce lactic acid of at least 10 times the amount produced by the wild type strain, when cultured under aerobic conditions. Also provided are methods to produce acetoin and 2,3-BDO.
Public/Granted literature
- US20190233907A1 BACTERIAL STRAIN PRODUCING 2,3-BUTANEDIOL AND OTHER METABOLITES Public/Granted day:2019-08-01
Information query
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