Invention Grant
- Patent Title: Method of identifying sequence variants using concatenation
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Application No.: US16693042Application Date: 2019-11-22
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Publication No.: US11111524B2Publication Date: 2021-09-07
- Inventor: Robert Osborne , Esther Musgrave-Brown
- Applicant: Oxford Nanopore Technologies, Ltd.
- Applicant Address: GB Oxford
- Assignee: Oxford Nanopore Technologies, Ltd.
- Current Assignee: Oxford Nanopore Technologies, Ltd.
- Current Assignee Address: GB Oxford
- Agency: Bozicevic, Field & Francis LLP
- Agent James S. Keddie
- Priority: GB1515558 20150902
- Main IPC: C12Q1/6827
- IPC: C12Q1/6827 ; C12Q1/6869 ; G16B30/00

Abstract:
Described herein, among other things, is a method of sequencing, comprising: concatenating a plurality of fragments of genomic DNA to produce concatenated DNA; sequencing the concatenated DNA to produce a plurality of sequence reads, wherein at least some of the sequence reads comprise: at least the sequence of the 3′ and/or 5′ ends of a fragment that corresponds to the locus of interest and sequence of one or both of the fragments that flank the fragment in the concatenated DNA; and grouping the sequence reads that corresponds to the locus of interest using, for each of the grouped sequence reads: the 3′ and/or 5′ end sequences; and/or the flanking sequence.
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