A banknote stacking and separating apparatus includes a frame, and a guide plate and a pressing plate that are disposed on the frame. The frame is provided with an entrance-and-exit for a banknote. One end of the guide plate adjacent to the entrance-and-exit is pivotally connected to the frame, and the guide plate is operative to rotate around an axis of a pivoting shaft and is configured to guide a moving direction of the banknote when the banknote is being collected. The pressing plate is configured to support the collected banknotes and press the banknotes towards the guide plate while the banknotes are being separated. One end of the guide plate far away from the entrance-and-exit is provided with a force-releasing surface configured to support the guide plate when the pressing plate presses the banknotes towards the guide plate.