The present disclosure provides a display substrate, a method for preparing the same, and a display device. The display substrate includes: a base substrate; a display function layer located on the base substrate, a first groove arranged in the first surface, and a first connection sub-line located in the first groove and covering a bottom and each side wall of the first groove, the first connection sub-line being connected to a signal input terminal; an integrated circuit located on a second surface, a second groove arranged in the second surface, and a second connection sub-line located in the second groove, the second connection sub-line being connected to the first connection sub-line and a signal output terminal of the integrated circuit.