Disclosed are a driving method, a driving device and a driving device of a display panel. The method comprises the following steps of: performing positive driving on a first adjacent sub-pixel of the two adjacent sub-pixels in a second direction in a first time interval, and performing negative driving on a second adjacent sub-pixel of the two adjacent sub-pixels in a second direction in a second time interval, wherein the duration of the first time interval is different from that of the second time interval. Obviously, due to the difference between the positive driving duration and the negative driving duration, the charging ability is different, forming a high-low voltage pixel unit interspersed arrangement, thus improving the color shift.