An insertion and plugging protection device includes: at least one first interface assembly; at least one second interface assembly; at least one connector, a first end of each connector being adapted to a corresponding first interface assembly, and a second end of each connector being adapted to a corresponding second interface assembly; a protection circuit forming a protection loop, the protection loop being configured to generate a preset electric signal when the first end of each connector is connected to the corresponding first interface assembly and the second end of each connector is connected to the corresponding second interface assembly; and a power source control circuit connected to the protection circuit and a power source module of the display device, and configured to control the power source module to output a first preset power source voltage signal in accordance with the preset electric signal.