A battery having a protective isolation structure is disclosed comprising a first current collecting layer, a first active material layer, a spacer layer, a first glue frame, a second active material and a second current collecting layer. The first active material layer is disposed on the first current collecting layer. The spacer layer is disposed on the first active material layer. The area of the spacer layer is smaller than the area of the first active material layer so that a part of the first active material layer is exposed outside the spacer layer. The first glue frame is covering the top surface of the first active material layer exposed from the spacer layer and has a protrusion disposed on the surface of the spacer layer. The second active material layer is disposed on the surface of the spacer layer and the protrusion. The second current collecting layer is disposed on the second active material layer.