A system including a processor, and memory having stored thereon instructions that, when executed by the processor, control the processor to receive image data of a sequence of images, and a current image of the sequence of images being after a previous image in the sequence of images, each of the current and previous images including a cell, filter the current image to remove noise, iteratively deconvolve the filtered current image to identify edges of the cell within the current image based on determined edges of the cell within the previous image, and segment the deconvolved current image to determine edges of the cell within the current image.