The method of manufacturing a secondary battery includes a layering step of forming an electrode body in which positive electrode plates and negative electrode plates are alternately layered with separators interposed in between, the layering step includes, a step of preparing a negative electrode sheet having negative electrode active material layers formed on two surfaces of a negative electrode core body, a step of forming a layered sheet by adhering a first separator and a second separator on two surfaces of the negative electrode sheet with adhesion layers in between, the layered sheet including the first separator, the negative electrode sheet, and the second separator, a step of forming a layered body by cutting the layered sheet, the layered body having two surfaces of the negative electrode plates sandwiched between the first and second separators, and a step of forming the electrode body using the layered body.