The present invention relates to a plant factory, which provides the following effects. The mesh-shaped floor is formed through the layer division support frame in the cultivation chamber, and the floor is divided into the cultivation layers having a multi-layered structure of two or more layers, thereby minimizing input resources and maximizing space and energy utilization efficiencies. In addition, a horizontal airflow is generally formed in each of the cultivation layers divided by the cultivation chamber air circulation supply unit, and an interlayer circulation airflow is formed between the respective cultivation layers divided by the mesh-shaped floor through the interlayer air circulation unit, and the cultivation table air supply unit creates a planting layer vertical descending air flow divided inside the cultivation table, which evenly improves the airflow rate regardless of the place in the cultivation chamber, reducing the deviation in temperature and carbon dioxide (CO2) concentration, and the net photosynthetic rate and plant may increase productivity by increasing the speed of growth.