Systems and methods are provided for performing OCT vibrography based on the synchronization of components of the OCT vibrography system. An A-scan trigger is employed to synchronize the operation of the scanning subsystem that scans the sample beam and an acoustic stimulus source that generates an acoustic stimulus for vibrographic measurements. The acoustic stimulus source is controlled such that when the scanning subsystem dwells on an imaging line selected for vibrography measurements, the acoustic stimulus is generated over a plurality of A-scans and the phase of the acoustic stimulus is locked to the A-scan trigger, such that the phase of the acoustic stimulus is incrementally modified with each A-scan. The accumulation of the acoustic phase is therefore synchronized to the A-scan trigger. The synchronization, providing synchronized acoustic phase evolution during each acoustic phase waveform cycle, permits the use of the OCT vibrography system for simultaneous anatomical and functional imaging.