A microfluidic device for the separation and immobilization of one or more cells into sample wells based on one or more physical properties of the one or more cells is provided. Metallic film or magnets are positioned on or below the wells. Openings in the device above the sample wells accommodate a measurement system to determine one or more physical characteristics or properties of the one or more cells immobilized within the microfluidic device. A method for determining a property or one or more physical characteristics of the immobilized one or more cells is also provided.