A system for neutralization of a target aerial vehicle comprises one or more counter-attack unmanned aerial vehicles (UAVs) and an aerial vehicle capture countermeasure tethering the counter-attack UAV(s) to a structure or ground. The counter-attack UAV(s) are operable to capture and neutralize the target aerial vehicle with the aerial vehicle capture countermeasure (e.g., a net). The system can comprise an on-board aerial vehicle detection system, and/or an external aerial vehicle detection system each having at least one sensor configured to detect the target aerial vehicle while in flight. The counter-attack UAV(s) and the associated detection systems may be autonomously operated for detecting and neutralizing a target aerial vehicle. The aerial vehicle capture countermeasure can be moved from a stowed position to a deployed position in response to coordinated flight of the counter-attack UAV(s). Associated methods and systems are provided.