A system and a related method for forming a multi-chamber package are disclosed. The system includes a single web of a film material extending in a machine direction and defining a plurality of lateral lanes extending contiguously in a cross-machine direction, orthogonal to the machine direction. The system also includes a forming arrangement configured to interact with the web to form chambers along a second lane of the plurality of lateral lanes in the machine direction, a first filling device configured to deposit a first substance into one or more of the formed chambers of the second lane, and a first folding mechanism configured to form a fold between the lateral lanes in the machine direction such that a first lane of the plurality of lateral lanes is directed to overlie the one or more formed chambers of the second lane.