Embodiments of information handling systems (IHSs) and methods are provided herein to automatically detect failure(s) on one or more power rails provided on a system motherboard of an IHS. One embodiment of such a method may include determining if a power rail test should be performed each time an information handling system (IHS) is powered on or rebooted. If a power rail test is performed, the method may perform a current measurement for each of the power rails separately to obtain actual current values for each power rail, compare the actual current values obtained for each power rail to expected current values stored for each power rail, and detect a failure on at least one of the power rails if the actual current value obtained for the at least one power rail differs from the expected current value stored for the at least one power rail by more than a predetermined percentage or amount.