A pixel arrangement structure, a display substrate and a display device. The pixel arrangement structure includes: a plurality of first sub-pixels and a plurality of sub-pixel groups arranged in an array, wherein the plurality of first sub-pixels and the plurality of sub-pixel groups are alternately arranged along a first direction to form pixel rows, and are alternately arranged along a second direction intersected with the first direction to form pixel columns; each of the plurality of sub-pixel groups includes a second sub-pixel, a third sub-pixel and another second sub-pixel sequentially arranged along the first direction; in the same pixel row, a ratio of a distance between the geometric centers of each second sub-pixel and the adjacent third sub-pixel to a distance between the geometric centers of each first sub-pixel and the third sub-pixel in the adjacent sub-pixel group is greater than or equal to ¼ and less than ½.