A display structure and a manufacturing method thereof are provided. The manufacturing method including steps of: (a) providing a carrier sequentially including a rigid substrate, a sacrificial layer, and a graphene layer; (b) forming a flexible substrate on the carrier; (c) forming a display component layer on the flexible substrate; and (d) removing the rigid substrate and the sacrificial layer. By using the sacrificial layer and the graphene layer to prevent display structures from being damaged by a laser peeling off step and protect the flexible substrate during the manufacturing process, and increase the bendability of the flexible substrate.