Disclosed is an operation amplification circuit and an over-current protection method therefor. The operation amplification circuit comprises: a control unit, configured to generate an output control signal according to an input signal and an output signal; an output unit, configured to generate an output current under control of the output control signal, wherein the output unit comprises an output capacitor which is charged or discharged by the output current to generate the output signal; an over-current protection unit, obtaining a temperature control current according to an operating temperature of the operation amplification circuit, wherein when the operating temperature is greater than or equal to a predetermined temperature, the temperature control current is positively correlated with the operating temperature, and the over-current protection unit adjusts the output control signal according to the temperature control current to limit the output current.