An electronic device according to an embodiment may include a housing, a display exposed through at least part of a first surface of the housing, an image sensor, a processor operatively connected with the display and the image sensor, and a memory operatively connected with the processor, wherein the memory may store instructions that, when executed, cause the processor to detect a touch of a finger of a user on the image sensor, while displaying a user interface using the display, in response to detecting the touch, while the touch of the finger is maintained on the image sensor, change the user interface in an area of the display within a preset distance from the image sensor, and acquire biometric information of the user based on reflection light of light emitted from the display, where the reflection light is acquired using the image sensor.