The present disclosure provides a microfluidic chip, a device and a method for chemiluminescence immunoassay. The microfluidic chip includes a base plate, as well as a first liquid inlet channel, a second liquid inlet channel, a third liquid inlet channel, an immune reaction cell, and a luminescent reaction cell formed on a first surface of the base plate. An outlet end of the immune reaction cell is in communication with a liquid inlet end of the luminescent reaction cell, and a primer is disposed in the immune reaction cell for immobilizing an antigen-antibody complex generated in the immune reaction cell.