Invention Grant
- Patent Title: Production method for nerve tissue
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Application No.: US15521334Application Date: 2015-10-23
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Publication No.: US11214771B2Publication Date: 2022-01-04
- Inventor: Atsushi Kuwahara , Suguru Yamasaki , Yasushi Hiramine , Yoshiki Sasai , Masayo Takahashi
- Applicant: SUMITOMO DAINIPPON PHARMA CO., LTD. , RIKEN , SUMITOMO CHEMICAL COMPANY, LIMITED
- Applicant Address: JP Osaka; JP Wako; JP Tokyo
- Assignee: SUMITOMO DAINIPPON PHARMA CO., LTD.,RIKEN,SUMITOMO CHEMICAL COMPANY, LIMITED
- Current Assignee: SUMITOMO DAINIPPON PHARMA CO., LTD.,RIKEN,SUMITOMO CHEMICAL COMPANY, LIMITED
- Current Assignee Address: JP Osaka; JP Wako; JP Tokyo
- Agency: Leydig, Voit & Mayer, Ltd.
- Priority: JPJP2014-217867 20141024
- International Application: PCT/JP2015/080016 WO 20151023
- International Announcement: WO2016/063985 WO 20160428
- Main IPC: C12N5/0793
- IPC: C12N5/0793 ; C12N5/0797 ; A61K35/30 ; A61L27/00 ; A61K35/12 ; C12Q1/02 ; C12N5/079 ; A61L27/38 ; G01N33/50

Abstract:
The present invention provides a method for producing neural cells or a neural tissue, including the following steps (1)-(3): (1) a first step of culturing pluripotent stem cells in the absence of feeder cells and in a medium containing 1) a TGFβ family signal transduction pathway inhibiting substance and/or a Sonic hedgehog signal transduction pathway activating substance, and 2) a factor for maintaining undifferentiated state, (2) a second step of culturing the cells obtained in the first step in suspension to form a cell aggregate, and (3) a third step of culturing the aggregate obtained in the second step in suspension in the presence or absence of a differentiation-inducing factor to obtain an aggregate containing neural cells or a neural tissue.
Public/Granted literature
- US20170313976A1 PRODUCTION METHOD FOR NERVE TISSUE Public/Granted day:2017-11-02
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