Invention Grant
- Patent Title: Activatable CRISPR/CAS9 for spatial and temporal control of genome editing
-
Application No.: US15559898Application Date: 2016-04-08
-
Publication No.: US11214779B2Publication Date: 2022-01-04
- Inventor: Alexander Deiters , James B. Hemphill , Aravind Asokan , Erin Borchardt
- Applicant: University of Pittsburgh—Of the Commonwealth System of Higher Education , The University of North Carolina at Chapel Hill
- Applicant Address: US PA Pittsburgh; US NC Chapel Hill
- Assignee: University of Pittsburgh—Of the Commonwealth System of Higher Education,The University of North Carolina at Chapel Hill
- Current Assignee: University of Pittsburgh—Of the Commonwealth System of Higher Education,The University of North Carolina at Chapel Hill
- Current Assignee Address: US PA Pittsburgh; US NC Chapel Hill
- Agency: Klarquist Sparkman, LLP
- International Application: PCT/US2016/026753 WO 20160408
- International Announcement: WO2016/164797 WO 20161013
- Main IPC: C12N9/00
- IPC: C12N9/00 ; C12N9/22 ; C12N15/10 ; C12N15/52 ; C12N15/85 ; A61K48/00 ; C12N13/00 ; C12N15/90
Abstract:
Disclosed herein is a genetically encoded light- or chemically-activated Cas9 engineered through the site-specific installation of an activatable lysine amino acid. Such activatable Cas9 proteins can be used in CRISPR/Cas9 systems to control gene expression temporally, spatially, or both. Systems, methods, kits, and compositions for manipulation of sequences and/or activities of target sequences are provided. Also provided are methods of directing CRISPR complex formation in cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas9 system.
Public/Granted literature
- US20180073002A1 ACTIVATABLE CRISPR/CAS9 FOR SPATIAL AND TEMPORAL CONTROL OF GENOME EDITING Public/Granted day:2018-03-15
Information query
