The present disclosure provides a touch screen and a manufacturing method thereof, a display substrate, and a touch display device. The touch screen includes: at least one metal mesh layer and electrostatic leading lines, wherein each metal mesh layer includes a plurality of electrodes insulated from each other, and the electrostatic leading lines extend among the plurality of electrodes of the at least one metal mesh layer and are connected to grounding wires, wherein the electrostatic leading lines are arranged to be insulated from the plurality of electrodes.