An Intelligent Real-Time Robot Operating System (IRT-ROS) architecture and an operation method thereof are provided. The IRT-ROS architecture includes a General-Purpose OS kernel, a Real-Time OS kernel, and an Inter-processor Interrupt interface. The General-Purpose OS kernel is configured to run a General-Purpose OS to execute a non-real-time process. The Real-Time OS kernel is configured to run a Real-Time OS to execute a real-time process. The IPI interface is connected between the General-Purpose OS kernel and the Real-Time OS kernel, and is configured to support communication between the non-real-time process and the real-time process. The AIRT-ROS architecture allows Linux and RTERS to respectively execute non-real-time process and real-time process, and to respectively respond IRQ of non-real-time devices and IRQ of real-time devices. Communications between non-real-time process and real-time process are supported. Therefore, the workload for driving the general-purpose external devices is greatly reduced.