A display device failure detection method, device and system are disclosed. The display device failure detection method includes that: at least one detection picture image acquired by an image acquisition device when a target display device regularly displays a detection picture sequence is received, and at least one image to be detected is determined from the at least one detection picture image (S11); at least one region to be detected corresponding to at least one display region of the target display device in the at least one image to be detected is determined, and image data of the at least one region to be detected is processed to obtain at least one image processing result (S13); and whether a display exception occurs to the target display device is judged according to the image processing result (S15).