A method and a control chip for performing access control of a memory device are provided, wherein the control chip is coupled to a host device. The method includes: utilizing a first transmission interface of the control chip to determine whether the memory device supports a second transmission interface different from the first transmission interface to generate a determination result; and according to user permissions of a user regarding the host device, determining whether to allow the control chip to decide whether to utilize the second transmission interface to access the memory device based on the determination result. In addition, if the user permissions satisfy a predetermined condition, a user interface of the host device may display a pop-up window in order to allow the user to decide which one of the first transmission interface and the second transmission interface to utilize for accessing the memory device.