Various methods and systems are provided for determining and characterizing features of an anatomical structure from a medical image. In one example, a method comprises acquiring a plurality of medical images over time during an exam, registering the segmented anatomical structure between the plurality of medical images, segmenting an anatomical structure in a one of the plurality of medical images after registering the plurality of medical images, creating and characterizing a reference region of interest (ROI) in each of the plurality of medical images, determining characteristics of the anatomical structure by tracking pixel values of the segmented and registered anatomical structure over time, and outputting the determined characteristics on a display device.